Homework

Weekly homework submissions:

• Week 1 HW: Principles and Practices

  1. Describe a biological engineering application or tool you want to develop and why At my lab, we came across the problem that, after doing the analysis of the best peptides of interest, we're unable to correctly asses the complete presentation of such peptides in the trained T cells, and we don't know if it is because of the lack of translation (peptide is not being produced), or if it's a lack of presentation (peptide is not going through the ER to an MHC, or not going through the Golgi apparatus to the outside of the cell). Thus, it would be very usefull to be able to use a circuit to express fluorescence when peptide is presented, and also, inside the cell, to know with fluorescence microscopy, the specific location of the peptide if it's inside the cell, or just not at all translated. This is also a general problem we're finding in T Cell Therapy, and could also target the problem where we don't usually have standards for Mass Spec analysis. In views of this problem and, after a little bit of research, I found a good inspiration paper.

• Week 2 HW: DNA Reading, writing and editing

  DNA reading, editing and design