https://pages.htgaa.org/2026a/keerthana-gunaretnam/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly Answer these questions about the protocol in this week’s lab What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix is a pre-formulated reagent designed to streamline PCR setup while delivering accurate, high-yield amplification. It bundles all the core enzymatic and chemical components needed for PCR into a single 2X concentrated solution, so the only additions needed are primers, template, and water. The central component is Phusion DNA Polymerase, a thermostable enzyme derived from a archaeal source and engineered for exceptional fidelity. Unlike standard Taq polymerase, Phusion carries a 3’ to 5’ exonuclease (proofreading) activity that corrects misincorporated nucleotides during synthesis, resulting in an error rate roughly 50 times lower than Taq. This is especially critical in this lab because any unintended mutations outside the chromophore region could compromise protein folding or color expression.Hugoen