<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Gibson Assembly Lab :: 2026a-kshitij-sodani</title><link>https://pages.htgaa.org/2026a/kshitij-sodani/labs/gibson-assembly-group-lab/index.html</link><description>Week 6 Lab: Gibson Assembly This lab focused on using PCR, Gibson Assembly, and bacterial transformation to modify a plasmid carrying the amilCP chromoprotein gene. The goal was to introduce targeted color mutations and then transform the assembled plasmids into E. coli so that successful variants could be identified by colony color.
Our group worked on color variants intended to produce magenta and blue colonies. The experiment had several stages: PCR amplification, PCR cleanup, diagnostic gel electrophoresis, Gibson Assembly, transformation, recovery, plating, and colony observation.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/kshitij-sodani/labs/gibson-assembly-group-lab/index.xml" rel="self" type="application/rss+xml"/></channel></rss>