Labs
Lab writeups:
Week 11 Lab: Cloud Lab
title: ‘DNA Gel Art’ weight: 10 Week 2 Lab: DNA Gel Art This lab was about using restriction digests and gel electrophoresis to make DNA gel art. Instead of only using a gel as a diagnostic tool, we used the positions of DNA bands as a visual medium. The basic idea was that different restriction enzymes cut Lambda DNA into different fragment sizes, and those fragments separate into bands when run through an agarose gel.
Week 6 Lab: Gibson Assembly This lab focused on using PCR, Gibson Assembly, and bacterial transformation to modify a plasmid carrying the amilCP chromoprotein gene. The goal was to introduce targeted color mutations and then transform the assembled plasmids into E. coli so that successful variants could be identified by colony color. Our group worked on color variants intended to produce magenta and blue colonies. The experiment had several stages: PCR amplification, PCR cleanup, diagnostic gel electrophoresis, Gibson Assembly, transformation, recovery, plating, and colony observation.
Neuromorphic Circuits and Biomaterials
I was not able to attend this lab in person because I was traveling for an interview. I informed Ronan ahead of time. A makeup session was not offered because this lab depended on time-sensitive biological materials, including mammalian cells that had already been grown or plated for the scheduled experiment. Even though I missed the hands-on part, I reviewed the lab materials and thought through what I would have done if I had been there.
Photos
Dialga: A Legendary Pokemon from the Sinnoh region