Week 2 HW.4: Twist DNA Synthesis Order

Steps to build a plasmid:

• Import DNA into Benchling.
• Add promoter, RBS, start/stop codons, 7x His Tag, and terminator
• Export .fasta and import into Twist.
• Order Twist clonal gene, using pTwist Amp High Copy vector.
• Export .gb (genbank) file for plasmid.
• Import plasmid .gb file into Benchling, open Info>Toplogy and set Circular.