https://pages.htgaa.org/2026a/libi-matityahoo/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly Questions What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains several key components that enable accurate and efficient DNA amplification. First, it includes the Phusion DNA polymerase, which is responsible for synthesizing new DNA strands. This enzyme has both 5’→3’ polymerase activity and 3’→5’ exonuclease (proofreading) activity, which allows it to correct mistakes during DNA replication and results in very high fidelity compared to standard polymerases like Taq. The mix also contains deoxynucleotides (dNTPs), which are the building blocks used by the polymerase to construct the new DNA strands. In addition, it includes an optimized reaction buffer with MgCl₂, which provides the proper chemical environment for the enzyme to function. Because all of these components are already included in the master mix, only the template DNA, primers, and water need to be added to perform the PCR.Hugoen