https://pages.htgaa.org/2026a/libi-matityahoo/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Answer: Cell-free protein synthesis offers greater flexibility and control compared to traditional in vivo methods because the reaction occurs outside of living cells. This allows precise control over experimental variables such as temperature, pH, ion concentrations, and the addition of specific components (e.g., cofactors, inhibitors, or non-natural amino acids), without being constrained by cellular viability or stress responses. Additionally, cell-free systems are not time-dependent on cell growth, making them faster and more consistent. DNA constructs can also be prepared and stored for long periods and used on demand.Hugoen