https://pages.htgaa.org/2026a/libi-matityahoo/labs/week-06-lab-gibson-assembly/index.htmlIn this week’s 2-day lab, we learned how to construct a plasmid using Gibson assembly and genetically engineer E. coli. Our goal was to mutate the chromophore of amilCP, a purple chromoprotein originally from the coral Acropora millepora, to generate new color variants. Using PCR with mutation-introducing primers, we modified the gene and assembled it into a plasmid containing the elements needed for replication and expression in bacteria. The plasmid was then transformed into E. coli so the cells could produce the engineered protein.Hugoen