https://pages.htgaa.org/2026a/likhitha-narapureddy/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Ans: Cell-free protein synthesis offers significant advantages over in vivo methods due to its open and controllable nature. It allows direct manipulation of reaction components, precise control over parameters such as pH and substrate concentration, and eliminates constraints related to cell viability. As a result, all system resources can be directed toward protein production, enabling rapid optimization and high-throughput experimentation.Hugoen