https://pages.htgaa.org/2026a/louisa-zhu/homework/week-10-hw-imaging-and-measurement/index.htmlPart 1: Final Project To validate my “Aragonite Anchor” this semester, I’ll first confirm protein expression and localization using SDS-PAGE and Western Blotting. By fractionating the cells and targeting my specific gene tags, I can prove the OmpA-CBP-1 fusion is actually reaching the outer membrane. To measure the binding affinity to CaCO3, I’ll use Fluorescence Spectroscopy; by tagging the protein with a marker like GFP, I can quantify exactly how much “glue” stays stuck to the crystals after washing.For structural characterization, I’ll use XRD to verify I’ve successfully formed the aragonite phase and SEM to get the “money shot” of the layered, nacre-like morphology. Finally, I’ll test the mechanical strength of the composite via Nanoindentation. This will let me measure the Young’s Modulus and fracture toughness, providing the quantitative data needed to prove my engineered protein actually makes the material stronger than a standard mineral control.Hugoen