Labs
Lab writeups:
Overview Materials: The following items were used in the lab: P200 Pipette: 20-200uL Pipette tips Eppendorf tubes PCR Tubes Petri dish Sharpie Tube holder invitrogen E-Gel EX: Agarose 1% Solutions I used included: Blue & Red food dye solutions DNA ladder solution (I was not able to get the exact details as the photo of the tube I took was blurry) H2O Machines I used included:
Overview Materials: The following items were used to prepare the agarose gel: Microwavable media storage bottles Agarose TAE buffer SYBR Safe DNA stain Gel tray & comb Eppendorf tubes PCR Tube rack Blue light transilluminator Imaging device Biological material I used included:
Overview Pre-Lab Process The idea of having to write my own code to create this art sounded terrifying at first given that I probably barely passed 6.100A. Then I found out a lot of it was written in Google Colab and felt relieved, until I kept running into issues so I decided to give up and just use Ronan’s code from his website: https://opentrons-art.rcdonovan.com/
Week 4 HW: Protein Design Part I
This lab is embedded into Part 3 of my Homework for Week 4
Day One Materials: The following items were used: PCR tubes Centrifuge tubes P200 pipette with 200uL tips P20 pipette with 20uL tips Nuclease-free water Sharpie Tube holder invitrogen E-Gel EX: Agarose 1% Biological material I used included:
Protocol For this section, I had to first download Neuromorphic Wizard, which was a whole process but I managed. I just filled out the Genetic Circuit Design Template with a design of my choice: My group mates decided to do something pretty similar, so we went with the same overall ERN and ERN_rec_ERNs. These were the predictions and experimental set-up that Neuromorphic Wizard came up with.