Week 1 Lab: Pipetting

Overview

Materials:

The following items were used in the lab:

• P200 Pipette: 20-200uL
• Pipette tips
• Eppendorf tubes
• PCR Tubes
• Petri dish
• Sharpie
• Tube holder
• invitrogen E-Gel EX: Agarose 1%

Solutions I used included:

• Blue & Red food dye solutions
• DNA ladder solution (I was not able to get the exact details as the photo of the tube I took was blurry)
• H2O

Machines I used included:

• invitrogen E-Gel PowerSnap

Pipette Art

In this part, I created artworks using food dye solutions in a petri dish. The methodology was as follows:

1. Grab a petri dish and draw a design (in my case, flowers with some geometric components).
2. Attach pipette tip to the pipette
3. Draw 150 uL of chosen solution into pipette
4. Pipette out droplets in pattern and shapes of choice
5. Dispose of tip when finished
6. Repeat steps 2-5 until satisfied
7. Admire artwork!

Gel Electrophoresis

In this part, I tried running an electrophoresis machine for the first time! My methodology was as follows:

1. Turn on invitrogen E-Gel PowerSnap
2. Remove invitrogen E-Gel EX: Agarose 1% from packet
3. Assemble package into machine
4. Attach pipette tip to the pipette
5. Open PCR tube of DNA ladder solution
6. Draw 15 uL of DNA ladder Solution
7. Pipette out complete volume into chosen wells
8. Dispose of pipette tip when finished and attach a new one
9. Draw 15 uL of H2O
10. Pipette out complete volume into remaining wells
11. Close lid of machine
12. Run for 15 minutes
13. Admire the process!

Pictures