https://pages.htgaa.org/2026a/madeline-eibner-gebhardt/homework/week-08-txtl-systems/index.htmlGeneral HW Questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis allow the expression of systems that might otherwise be toxic to or impose a high level of burden upon a living cell. The lack of native cellular pathways (which often respond to circuit presence in unexpected ways and can sometimes compromise the functioning of a circuit) also means that the researcher need not fear interference or complications due to the behavior of the host. Cell free methods are more beneficial than cell production for working with sensing/detection constructs such as riboswitches, since (in the absence of a cell membrane) the construct will function better with direct access to compounds of interest in the environment. Cell free methods are also advantageous when studying systems that would otherwise kill or significantly reduce the functioning of the host cell – e.g., toxins or high-burden circuits.Hugoen