https://pages.htgaa.org/2026a/madeline-eibner-gebhardt/labs/week-06-lab-assemblyi/index.htmlPCR Setup Purple and Orange NFW – added fwd - Pu, added fwd - O, added rev – same for both, added Master mix – added Template plasmid – added Calculated PCR params and started running Need to add DPN1 to digest the template DNA from the PCRs for w/out template DPN1 currently at -20 BEL – thaw for a bit 1 ul DPN1 to ea rxn (put back into freezer)Hugoen