Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
- Biological Engineering Application Proposed application: Engineered microbial biofactories for small-molecule drug production. Having a background in Biomedical Science and current training in translational physiology and pharmacology, I am particularly interested in small-molecule drug development. This project proposes engineering commercially viable cells capable of producing small-molecule drugs that are difficult or costly to synthesize using traditional chemical methods.
Week 2 HW: DNA Read, Write, & Edit
Part 1: Benchling & In-silico Gel Art This is the Lambda Sequence This is the Lambda sequence with the cuts First, I tried to use Ronan’s website to get a template, and then I made it in Benchling Template: Benchling: 3.1. Choose your protein. I decided to use the Kappa Opioid GPCR, as this is the target for my biofactory, which is for my final project. It comes from the OPKR1 gene, and UniProt entry P41145 (OPRK_HUMAN) lists the canonical human kappa opioid receptor as 380 amino acids with this sequence:
Homework Submission Design Explanation For the art, I first sketched ideas on my tablet and then tried to upload them as an image on OpenTrons. The image with birds flying through the sky was pleasing to me aesthetically, but there were limited colours available to us. I chose the following palm tree and adapted some of the colours.
Week 4 HW: Protein Design Part I
Protein Design Part I (Thras Karydis, Jon Kaufman)
Lab: Protein Design IWeek 5 HW: Protein Design Part II
Protein Design Part II (Pranam Chatterjee, Gabriele Corso)
Lab: Protein Design IIWeek 6 HW: Genetic Circuits Part I
Genetic Circuits Part I: Assembly Technologies (Doug Densmore, Traci Haddock)
Lab: Gibson AssemblyWeek 7 HW: Genetic Circuits Part II
Genetic Circuits Part II: Neuromorphic Circuits (Ron Weiss, Evan Holbrook)
Lab: Neuromorphic CircuitsWeek 10 HW: Advanced Imaging & Measurement Technology
Advanced Imaging & Measurement Tech (Evan Daugharthy, Waters Corp.)
Mass Spectrometry





