https://pages.htgaa.org/2026a/maria-jose-bolivar/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly Answer these questions about the protocol in this week’s lab: What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? R/The Phusion HF PCR Master Mix contains several key components. The Phusion DNA Polymerase is a high-fidelity polymerase with 3’→5’ exonuclease (proofreading) activity, which significantly reduces errors during amplification. dNTPs (dATP, dTTP, dCTP, dGTP) serve as the building blocks for the new DNA strand. The HF Buffer stabilizes pH and ionic conditions optimal for standard templates, while the GC Buffer is used for GC-rich sequences. MgCl₂ acts as an essential cofactor for polymerase activity. Additional stabilizers and additives improve enzyme stability and overall reaction efficiency.Hugoen