<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 10 HW: Advanced Imaging and Measurement Technology :: 2026a-mariana-diaz-luna</title><link>https://pages.htgaa.org/2026a/mariana-diaz-luna/homework/week-10-hw-imaging-and-measurement/index.html</link><description>Homework: FINAL PROJECT
One of the main aspects to measure in this work is the expression level of the APP since the goal of the CRISPRi system is to reduce its transcription. This could be measured using qPCR (mRNA levels of APP), extracting the RNA from treated and control cells, performing reverse transcription and quantifying gene expression differences.
It is also important to measure whether changes in gene expression translate into protein level changes, using Western Blot to detect APP protein levels or ELISA to quantify amyloid-beta peptides.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/mariana-diaz-luna/homework/week-10-hw-imaging-and-measurement/index.xml" rel="self" type="application/rss+xml"/></channel></rss>