https://pages.htgaa.org/2026a/mariana-kanbe/homework/week-06-genetic-circuits-pt1/index.htmlAssignment: DNA Assembly Question 1: What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix is a 2X, ready-to-use mixture where the exact formulation is partly proprietary, but the functional components are documented in the manufacturer’s manual: Component (Phusion 2X Master Mix) Purpose Phusion High-Fidelity DNA Polymerase DNA synthesis with high fidelity + proofreading dNTPs (dATP, dCTP, dGTP, dTTP) Building blocks for new DNA strands HF reaction buffer (salts + pH buffer) Maintains optimal pH/ionic strength for enzyme function Mg2+ (via buffer system; often MgCl2-derived) Essential polymerase cofactor Stabilizers / additives (partly proprietary) Improve enzyme stability and consistency Nuclease-free water Solvent to reach correct 2X working concentrations Reference: Thermo Fisher Phusion High–Fidelity DNA Polymerase Product Information Sheet, standard biochemistry manuals (e.g., Sambrook & Russell).Hugoen