Labs

Lab writeups:

  • Week 1 Lab: Pipetting

    Committed Listeners: Not Applicable.

  • Week 2 Lab: DNA Gel Art

    For Week 2, the wet-lab component was optional for CLs with lab access, which unfortunately was not my case. I completed and documented the in-silico design and written assignments on my Homework Week 2 page.

  • Week 3 Lab: Lab Automation

    For Week 3, CLs were required to create and document the Opentrons Python script, answer the post-lab questions, and submit final project ideas. I completed the code-based assignment and documentation on my Homework Week 3 page, but I did not have access to run the script physically on an Opentrons robot.

  • Week 4 Lab: Protein Design Part 1

    Lab work this week is contained within my Homework Week 4 page.

  • Week 5 Lab: Protein Design Part 2

    Lab work this week is contained within my Homework Week 5 page.

  • Week 6 Lab: Gibson Assembly

    For this week, I completed what was expected from the CL side: the conceptual and design-oriented homework around PCR, Gibson cloning, transformation, Golden Gate Assembly, Benchling modeling, and Asimov Kernel exercises. Since I did not have access to a physical lab, I did not perform the wet-lab workflow, but my Week 6 Homework Documentation covers the main principles behind the lab assignment.

  • Week 7 Lab: Neuromorphic Circuits

    For this lab, the physical wet-lab component was not something I had access to as a CL. Kindly check my Week 7 Homework Documentation.

  • Week 9 Lab: Cell Free Systems

    For Week 9, I completed the required CL homework components, including the general cell-free systems questions, lecturer-specific questions, and final project planning. Kimdly check my Week 9 Homework page.

  • Week 10 Lab: Mass Spectrometry

    For Week 10, I completed the CL homework requirements based on the provided lab screenshots/data as allowed in the homework instructions. Kindly check my Week 10 Homework Documentation.

  • Week 11 Lab: Cloud Laboratories Homework & Lab

    As a CL without access to a physical lab, I completed the Week 11 cloud lab assignment through the design and documentation components. I contributed to the collective artwork, described the cell-free reaction components, compared the master mix strategies, and submitted reaction designs for the global experiment, focusing on mScarlet-I and sfGFP readouts with HEPES-KOH, tyrosine, and magnesium glutamate. Kindly check my documentation for Week 11 Homework.

  • Week 12 Lab: Bioproduction of Beta-Carotene and Lycopene

    Post Lab Questions (Mandatory for All Students)

  1. Which genes when transferred into E. coli will induce the production of lycopene and beta-carotene, respectively? According to the lab instructions, lycopene production in E. coli is induced by transferring the three genes from Erwinia herbicola: crtE, crtI, and crtB. These genes convert FPP into lycopene. Beta-carotene production uses the same pathway with the addition of crtY, which enables conversion toward beta-carotene.