https://pages.htgaa.org/2026a/mariia-moshchenskaia/homework/week-06-hw-genetic-circuits-part-i-assembly-technologies/index.htmlAssignment: DNA Assembly Components of the Phusion High‑Fidelity PCR Master Mix and their purposes The Phusion master mix (2×) contains: Phusion DNA polymerase – a high‑fidelity enzyme with 3′→5′ proofreading activity, minimising errors during amplification dNTPs (dATP, dTTP, dCTP, dGTP) – the building blocks for new DNA strands Optimised reaction buffer (HF or GC) – provides the correct pH and ionic conditions for the enzyme Mg²⁺ ions (MgCl₂) – an essential cofactor for polymerase activityHugoen