https://pages.htgaa.org/2026a/mariia-moshchenskaia/homework/week-11-hw-bioproduction--cloud-labs/index.htmlPart A: The 1,536 Pixel Artwork Canvas | Collective Artwork Collective image Part B: Cell-Free Protein Synthesis | Cell-Free Reagents Roles of components in the 20‑hour cell‑free reaction (yellow box) E. coli Lysate (BL21 (DE3) Star) – provides the cellular machinery (ribosomes, tRNAs, polymerases, translation factors) required for transcription and translation. The BL21 (DE3) strain supplies T7 RNA polymerase, enabling high‑efficiency transcription from T7 promoters. Salts/Buffer – maintain optimal pH (HEPES), ionic strength, and cofactor availability. Potassium glutamate and magnesium glutamate stabilise ribosomes and enzyme activity; potassium phosphate buffers the reaction and supplies phosphate groups.Hugoen