https://pages.htgaa.org/2026a/marisa-satsia/homework/week-06--genetic-circuits--part-i/index.htmlAcropora Millepora, Photo from Reefbuilders This week we learn core molecular biology tools and techniques for processing and assembling DNA, including PCR and Gibson Assembly. Here is the updated HTGAA2026 Gibson assembly lab protocol document. Homework PART A: PCR and DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix is a 2X, ready-to-use mixture where the exact formulation is partly proprietary, but the functional components are documented in the manufacturer’s manual:Hugoen