https://pages.htgaa.org/2026a/maximino-matus-ruiz/homework/week-06-hw-genetic-circuits-part-i/index.htmlPart I. Assignment: DNA Assembly Answer these questions about the protocol in this week’s lab: https://docs.google.com/document/d/e/2PACX-1vSqMqA8QkKJu9eOM310hXPj_28e5JKeDNEceKAWwG--3JV_9v510vQguSCiRqvxJVnQ2Gdx2btXpeDT/pub What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Using Copilot I got the following answere and did some editions: Phusion High‑Fidelity PCR Master Mix is a 2X ready‑to‑use mix designed to simplify PCR setup while providing extremely high fidelity. There are five componets for specific porpuses: Component Purpose Phusion DNA Polymerase High‑fidelity, proofreading DNA synthesis HF or GC Buffer Optimizes reaction conditions; HF for fidelity, GC for difficult templates MgCl₂ Required cofactor for polymerase activity dNTPs Nucleotide building blocks for DNA amplification DMSO (optional) Enhances amplification of GC‑rich or structured templates What are some factors that determine primer annealing temperature during PCR? Primer Annealing Temperature: The primer melting temperature is the estimate of the DNA-DNA hybrid stability and critical in determining the annealing temperature. Too high Ta will produce insufficient primer-template hybridization resulting in low PCR product yield. Too low Ta may possibly lead to non-specific products caused by a high number of base pair mismatches,. Mismatch tolerance is found to have the strongest influence on PCR specificity. *This information was taken from:*https://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.htmlHugoen