https://pages.htgaa.org/2026a/michael-yang/homework/week-06-genetic-circuits-part-i/index.htmlWhat are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The mix includes: Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. DNA Polymerase builds the DNA sequence from deoxynucleotides starting with the template strands and primer. It’s high-fidelity, so it won’t make as many errors as Taq. Reaction buffer allows the reaction to take place. What are some factors that determine primer annealing temperature during PCR? For primer annealing, we want the template to be high enough for the template DNA to be denatured and but low enough that some of the primer is annealed. So the lab protocol suggests to use temperatures near the Tm of the primer (temp where the DNA is 50/50 double/single stranded).Hugoen