Week 13 HW — Biodesign & Biofabrication

Pulse Space — Biological Direction & System Integration

## Biodesign & Biofabrication

This week focused on strengthening the biological and fabrication framework of Pulse Space.

Earlier stages of the project primarily investigated conceptual questions around temporal interiors and spatial memory. This week, the research shifted toward developing a more grounded biological system architecture capable of supporting controlled material transformation.

The project began exploring how synthetic biology, microfluidics, and hydrogel-supported systems could operate together as a distributed biological-material infrastructure.

Rather than treating biology as a decorative layer, the project investigates biology as a programmable and temporally regulated material system.


Current Biological Direction

The current biological direction investigates:

  • engineered non-pathogenic E. coli platform
  • inducible chromoprotein expression system
  • investigation of amilCP chromoprotein expression
  • hydrogel-supported bacterial chambers
  • sealed microfluidic activation environment

At this stage, the project remains exploratory and focuses on understanding how biological activation may be spatially and temporally regulated within interior material systems.


Biological Activation Logic

The project investigates how physiological conditions may be translated into biological transformation through a controlled activation workflow.

Physiological sensing
Threshold evaluation
Controlled inducer release
Microfluidic distribution
Chromoprotein activation
Pigment accumulation
Spatial memory formation