https://pages.htgaa.org/2026a/moola-mutondo/homework/week-06-hw-genetic-circuits-part-i/index.htmlWhat are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase - this is for amplifying the DNA by polymerisation. It is special because when used with its special buffer, it has up to 52× higher fidelity rate than ordinary Taq polymerase dNTPs- These are the building blocks that are used in the polymersiation by the DNA polymerase MgCl₂ - DNA polymerase enzyme needs magnesium ions to function properly (they are necessary cofactors for the thermostable enzyme) Optimized buffer - Phusion needs an optimized buffer because it uses a highly processive and high-fidelity DNA polymerase (originally a Pfu-like enzyme fused to a processivity domain) that is very sensitive to Mg²⁺, ionic strength, pH, and additives. Therefore these components must be balanced perfectly for the PCR not to fail in terms of processivity or fidelity. These items are added to the PCR: template DNA, primers, and molecular water. What are some factors that determine primer annealing temperature during PCR? Here’s the table simplified into bullet points:Hugoen