https://pages.htgaa.org/2026a/moola-mutondo/homework/week-09-hw-cell-free-systems/index.htmlGeneral Homework Questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. -Cell-free can be used even when cold-chain conditions cannot be assured -Cell-free systems can be used without cloning and creating recombinant organisms Cell-free protein synthesis offers flexibility and a lot of control because you can directly tune reaction components (DNA amount, salts/cofactors, energy mix, chaperones, redox environment) and test many conditions rapidly without cell growth, regulation, or viability constraints. Cell-free expression is especially beneficial for rapid prototyping of constructs/circuits and also producing products especially if they are tocix to living expression systems. It can also enable on-demand expression (e.g., freeze-dried systems) and reactions requiring tightly controlled chemistry. This is great for making vaccines on demand on a mission to Mars for example. Describe the main components of a cell-free expression system and explain the role of each component. For General Systems Cell extract (lysate) or purified translation system — supplies the machinery that makes protein (ribosomes, tRNAs, translation factors, enzymes).Hugoen