Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
First, describe a biological engineering application or tool you want to develop and why. This could be inspired by an idea for your HTGAA class project and/or something for which you are already doing in your research, or something you are just curious about. I want to develop a biological engineering application that uses directed evolution to develop super-enzyme(s) capable of converting cassava peels which are a rich, low-cost source of lignocellulosic waste into prebiotic oligosaccharides. Cassava peels contain Xylan (a type of hemicellulose) that, when broken down through alkali pretreatment and enzymatic hydrolysis, yields Xylooligosaccharides (XOS), which acts as a prebiotic by promoting the growth of beneficial gut bacteria and can be used for food and nutraceutical applications.
Week 2 HW: DNA Read, Write & Edit
Part 1: Benchling & In-silico Gel Art First I created an account on Benchling Then, I imported Escherichia phage Lambda, complete genome with GenBank ID: J02459.1 Fig 1: Linear map of lambda DNA (LAMCG) imported into Benchling. Next, I simulated restriction enzyme digestion with the following enzymes EcoRI HindIII BamHI KpnI EcoRV SacI SalI Fig 2: Simulating restriction enzyme digestion of lambda DNA on Benchling.