https://pages.htgaa.org/2026a/nana-agyei-afrane-asare/projects/project-idea-drafts/index.htmlColorimetric Cell-Free Biosensor for Heavy Metal Pollutant Detection Illegal artisanal and small-scale gold mining locally known as galamsey has devastated river systems across Ghana, particularly the Pra, Ankobra, Offin, and Birim rivers, contaminating drinking water sources with toxic heavy metals including lead (Pb), arsenic (As), mercury (Hg), and cadmium (Cd). Existing water quality monitoring infrastructure is sparse, expensive, and inaccessible to the rural communities most affected. This project proposes the computational design and in silico validation of a multiplexed, colorimetric, cell-free biosensor capable of simultaneously detecting all four heavy metals in field-collected water samples, with results readable by the naked eye against a printed color reference card, requiring no electricity, laboratory equipment, or smartphone.Hugoen