Week 11 HW: Building Genomes

I made this fish-like shape at the second quadrant (on the right side of the plate)

I liked that this project brings our creative side, and we use fluorescent protein expression to reflect artistry.

For next collaborative project there could be done in modified bacteria grown in different growth media (so as to see different color expression)

main differences between the 1-hour optimized PEP-NTP master mix and the 20-hour NMP-Ribose-Glucose master mix

PEP‑NTP starts with fully charged NTPs so this means translation begins instantly.
NMP‑Ribose‑Glucose: cells must first convert NMP → NDP → NTP, which takes time.
PEP-NTP: NTPs are expensive, needs pH buffers
NMP‑Ribose‑Glucose runs longer because glucose/ribose are abundant, cheap and slowly metabolized.
Note: Enables longer expression