Week 11 HW: Building Genomes
Part A: The 1,536 Pixel Artwork Canvas | Collective Artwork
I contributed 123 pixels to the global artwork experiment by making HTGAA letters at the bottom left. I liked the collaborative work and that it represents all of us. I think it can be better by not allowing the replacement of anyone’s work.
Part B: Cell-Free Protein Synthesis | Cell-Free Reagents
| Component | Role |
|---|---|
| E. coli Lysate | |
| • BL21 (DE3) Star Lysate (includes T7 RNA Polymerase) | |
| Salts/Buffer | |
| • Potassium Glutamate | |
| • HEPES-KOH pH 7.5 | |
| • Magnesium Glutamate | |
| • Potassium phosphate monobasic | |
| • Potassium phosphate dibasic | |
| Energy / Nucleotide System | |
| • Ribose | |
| • Glucose | |
| • AMP | |
| • CMP | |
| • GMP | |
| • UMP | |
| • Guanine | |
| Translation Mix (Amino Acids) | |
| • 17 Amino Acid Mix | |
| • Tyrosine | |
| • Cysteine | |
| Additives | |
| • Nicotinamide | |
| Backfill | |
| • Nuclease Free Water |