Week 11 HW: Building Genomes

Part A: The 1,536 Pixel Artwork Canvas | Collective Artwork

I contributed 123 pixels to the global artwork experiment by making HTGAA letters at the bottom left. I liked the collaborative work and that it represents all of us. I think it can be better by not allowing the replacement of anyone’s work.

pixels pixels

Part B: Cell-Free Protein Synthesis | Cell-Free Reagents

ComponentRole
E. coli Lysate
• BL21 (DE3) Star Lysate (includes T7 RNA Polymerase)
Salts/Buffer
• Potassium Glutamate
• HEPES-KOH pH 7.5
• Magnesium Glutamate
• Potassium phosphate monobasic
• Potassium phosphate dibasic
Energy / Nucleotide System
• Ribose
• Glucose
• AMP
• CMP
• GMP
• UMP
• Guanine
Translation Mix (Amino Acids)
• 17 Amino Acid Mix
• Tyrosine
• Cysteine
Additives
• Nicotinamide
Backfill
• Nuclease Free Water