https://pages.htgaa.org/2026a/nourelden-rihan/homework/week-10-hw-imaging-and-measurement/index.html𓃠 Week 10 Homework 𓃠 Homework: Final Project α-Pinene titer: quantity of our target molecule produced per chassis per carbon source. Measured by GC-MS with dodecane overlay extraction from 96-well deep plates. AgPS protein identity and mass: purified AgPS-His6 protein (via Ni-NTA) can be characterized by intact protein LC-MS to confirm correct molecular mass and verify the His6 tag is present. Cell growth (OD₆₀₀): optical density at 600nm across all chassis × carbon source conditions. Measured on the PHERAstar FSX plate reader. Tells us if the strain is healthy while producing. Gene expression (mRNA level): RT-qPCR on the CFX Opus machine using primers targeting AgGPPS2 and AgPS transcripts. Confirms the genes are being transcribed in vivo. 𓋹 𓋹 𓋹 𓋹 𓋹 Homework: Waters Part I — Molecular Weight The Theoritical Molecular weight of the eGFP Sequence is 28006.60Hugoen