https://pages.htgaa.org/2026a/olga-mineyeva-2/homework/week-09-hw-cell-free-systems/index.htmlPart A: General and Lecturer-Specific Questions general questions Advantages of cell-free protein synthesis (CFPS) over in vivo methods Mainly, there is no living cell and no cell membrane involved, and so any component of the reaction can be added or removed in the course of the controlled reaction. There are fewer variables to control compared to an experiment and synthesis in a living cell. No transformation process is involved, and so the reaction is faster. Cell-free is more beneficial when a toxic protein is synthesized or a protein that incorporates non-canonical amino acids.Hugoen