Individual Final Project

Step One Prove lead-sensing logic

Aim: I am designing a modular genetic cassette in which a heavy-metal sensing element, likely pbrR/pbr, controls either a standard reporter or a geosmin output module, with HSR considered as an auxiliary stress-sensitive element rather than the primary detector

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Design intent: Detect bioavailable Pb(II) using a PbrR/pbr lead-responsive sensing module. Initial output will be a fluorescent reporter to validate sensing behavior. A later version may replace the reporter with a geosmin-associated output module for sensory repair signaling.

Cassette v1 should respond to bioavailable Pb(II) PbrR + pbr promoter/operator → reporter

Building aa Benching Circuit Template Promoter → pbrR → Terminator → pbr promoter → sfGFP → Terminator

Assuming E. coli as initial chassis for circuit implementation

References; https://www.nytimes.com/2026/04/29/science/nose-brain-smell-olfaction.html https://news.mit.edu/2017/bacteria-multicolor-vision-0526

Group Final Project