<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6: Genetic Circuits - part I :: 2026a-paulina-flores</title><link>https://pages.htgaa.org/2026a/paulina-flores/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly
Answer these questions about the protocol in this week’s lab:
What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose? Elements:
Pyrococcus-like enzyme fused with a processivity-enhancing domain that increases fidelity and speed. 53 polymerase activity, 35exonuclease activity, and generates blunt-ended products. Phusion DNA Polymerase, deoxynucleotides, and reaction buffer that has been optimized and includes MgCl2 Requirements to be added: template, primers, and water. Purpose:</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/paulina-flores/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>