https://pages.htgaa.org/2026a/pelin-onur/homework/week-05-hw-protein-design-part-ii/index.html✨ Part A. SOD1 Binder Peptide Design ✨ Part 1: Generate Binders with PepMLM Sequence Retrieval and Mutation I began by retrieving the human Superoxide dismutase 1 (SOD1) sequence from the UniProt database using the accession number P00441. The native (wild-type) sequence consists of 154 amino acids: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ To model the disease state, I introduced the ALS-causing A4V mutation (Alanine → Valine at residue 4). Noting that standard numbering excludes the initiator Methionine (M), I replaced the Alanine at the 5th position with a Valine to create my target mutant sequence:Hugoen