https://pages.htgaa.org/2026a/rahul-yaji/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase: Pyrococcus-like enzyme that contains a fused processivity-enhancing domain. It provides more than 50 gold higher fidelity than Taq polymerase. dNTPs: contains dATP, dCTP, dGTP, and dTTP that are required for extension reaction of the PCR. Buffers: MgCl2 as a cofactor for polymerase, KCl and TAPS-HCl ([tris(hydroxymethyl)methylamino]propanesulfonic acid) to maintain ionic strength and pH respectively, and beta-meracaptoethanol to maintain enzyme stability. Some other components that are provided seperately: DMSO (Dimethyl sulfoxide) to improve denaturation and primer binding, and nuclease free water as a solvent and matrix to avoid denaturation of the DNA. What are some factors that determine primer annealing temperature during PCR?Hugoen