https://pages.htgaa.org/2026a/raphael-aca/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions/answers Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis has several advantages over traditional in vivo protein expression methods. In cell-free systems, researchers have direct control over experimental conditions such as pH, ion concentrations, temperature, substrate availability, and DNA concentration without needing to maintain living cells. This allows for rapid optimization and easier manipulation of gene expression conditions. Cell-free systems also avoid problems associated with cellular toxicity, metabolic burden, and membrane transport limitations. In addition, proteins can be produced much more quickly because there is no need for cell growth or transformation. Cell-free expression is especially beneficial when producing toxic proteins that would kill living cells and when rapidly prototyping genetic circuits for synthetic biology applications.Hugoen