Homework
Weekly homework submissions:
I have answered the following questions to prepare for the lecture on DNA design and synthesis. These answers are based on the slides provided by Professor Jacobson, Dr. LeProust, and Professor Church. Question,1 Natures machinery for copying DNA is called polymerase. What is the error rate of polymerase? How does this compare to the length of the human genome. How does biology deal with that discrepancy?
Week 1 HW: Principles and Practices
ARM-Net: Alzheimer’s Recovery Micro-TNT Network 1.Biological Engineering Application I am developing ARM-Net, a synthetic biology tool designed to overexpress CCT4 specifically in microglia. Unlike the full TRiC/CCT complex, CCT4 can function independently or as homo-oligomers to promote microtubule synthesis. By boosting these chaperonins, I aim to induce the formation of Tunneling Nanotubes (TNTs) between microglia, creating an “Intercellular Care Network” (ICN)
Week 2: DNA Design and Characterization
This week, I focused on the design, simulation, and cloning workflow for CCT4, the core component of my project, ICN (Intercellular Care Network).
- Part 1: Benchling and In Silico Gel Art Benchling Simulation Having long been interested in Benchling, I used this assignment to dive deep into the tool. Instead of the suggested Lambda DNA, I worked with the CCT4 protein sequence relevant to my project. I simulated restriction digests using the following enzymes: