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The Only Question -RCBeck
Final Project Homework For your final project: Please identify at least one (ideally many) aspect(s) of your project that you will measure. It could be the mass or sequence of a protein, the presence, absence, or quantity of a biomarker, etc. Aim One: Measurment Data The first aim of this project transforms a strain of Synechococcus elongatus 7942 (PCC7942) into the sucrose secreting, model organism S. elongatus cscB+. The original PCC7942 colony will need to be grown to an optical density (OD) 750 of ≥1 before transformation may take place. A spectrophotometer will be used to obtain this measurement. - First growth media needs to be prepared, which will require measuring agar, BG11 medium, AS Trace Elements, water, pH, and temperature of growth media. Plates are poured, streaked and incubated. Colonies are then selected and transfereed to new grow media. - Colonies are incubated until an OD of 750 is reached, then natural transformation may take place. - Transformed samples are then recultured with spectinomycin treated plates, which selects for only transformed model organisms.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/robert-c-beck/homework/week-10-hw-advanced-imaging-technology/index.xml" rel="self" type="application/rss+xml"/></channel></rss>