There are several differnt size pipettes and tips to use depending on the amount of fluid you want to dispense or extract.
Pipettes sizes: P20: 1-20uL of liquid P200: 20-200uL P1000: 100-1000uL
Pipette tips: 10uL, 200uL, 1000uL
They are adjustable within their specified range using the adustment wheel located at top of pipette.
If you have a 1-20uL pipette and the set read out is: 015, then you have it set to aspirate/dispense 1.5uL.
The smallest tip will only fit on the small pipette.
There are are two stops on the plunger, the first stop measures the amount the pipette is set to, the second stop (plunger depressed all the way) will blow out the pipette.
There is a button on the top of the pipette which will detach/eject the tip from the pipette.
Tips are single use only!
Do not invert the pipette! This can damage the mechanism, especially if there is fluid in the tip. If there is no tip attached you can set it down.
Pipette Practice
Always pipette with a steady hand, and avoid touching the walls of Eppendorf or PCR tubes.
You can mix a solution by aspirating and dispensing samples in the tube. Do this slowly to avid introducing bubbles in the mixture.
