<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: DNA Assembly :: 2026a-robert-sheng</title><link>https://pages.htgaa.org/2026a/robert-sheng/homework/week-06-hw/index.html</link><description>DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity PCR Master Mix contains Phusion DNA Polymerase, dNTPs, reaction buffer, and MgCl₂. The DNA polymerase copies the DNA template during PCR. dNTPs are the building blocks used to synthesize the new DNA strand. The buffer keeps the reaction at the correct pH and salt condition. MgCl₂ is an important cofactor that helps the polymerase function properly. Since Phusion is a high-fidelity polymerase, it is useful when the amplified DNA sequence needs to be accurate for cloning or assembly.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/robert-sheng/homework/week-06-hw/index.xml" rel="self" type="application/rss+xml"/></channel></rss>