Labs

Lab writeups:

  • Week 1 Lab: Pipetting

  • Week 2_Lab: GelArt

  • Week 3_Lab: Opentrons Art

  • Week 4_Lab: Protein Design

    This week’s Lab work is effectively part of this week’s Homework;

  • Week 5_Lab: Protein Design Part II

    This week’s Lab work is effectively part of this week’s Homework;

  • Week 6_Lab: Gibson Assembly

    This week’s experiment was done late at night. To speed things up, we tried to simplify several steps in the protocol and cut a few corners, such as filtering only once when the protocol called for two rounds of filtration. In the end, we did not get the result we were hoping for. The plates only showed some unidentified white colonies, possibly E. coli, but the issue is that the original E. coli we used was supposed to be purple.

  • Week 7_Lab: Neuromorphic Circuits

    This week, I worked on designing a small genetic circuit system based on RNA endonucleases and fluorescent protein outputs. Instead of starting directly from a final circuit, we first organized the available biological parts and tried to understand how they could be connected into a regulatory network.

  1. Organizing the Basic Parts We started by making a spreadsheet of possible genetic circuit part names. The first group of parts was the RNA endonucleases, or ERNs: