Week 6_Lab: Gibson Assembly

This week’s experiment was done late at night. To speed things up, we tried to simplify several steps in the protocol and cut a few corners, such as filtering only once when the protocol called for two rounds of filtration. In the end, we did not get the result we were hoping for. The plates only showed some unidentified white colonies, possibly E. coli, but the issue is that the original E. coli we used was supposed to be purple.

So we took some samples from another group and tried again. This time, they grew successfully, which suggests that the problem most likely came from the way we modified or skipped parts of the protocol during the workflow.