https://pages.htgaa.org/2026a/sami-syed/labs/week-06-gibson-assembly/index.html🧬 PCR and Gibson Assembly Workflow In this two-day lab, we used PCR and Gibson Assembly to engineer mutations in the chromophore region of the purple Acropora millepora chromoprotein (amilCP) in order to generate a range of orange, pink, and blue colour variants. Two separate PCR reactions were performed to generate the DNA fragments required for Gibson Assembly. The insert PCR region extended from 24 base pairs upstream of the chromophore to just beyond the transcription terminator of the gene. The forward primer was specifically designed with an intentional mismatch to introduce a site-directed mutation into the mUAV plasmid DNA. After assembly, the mutated plasmids were transformed into chemically competent E. coli cells for expression and analysis of the resulting colour phenotypes.Hugoen