https://pages.htgaa.org/2026a/sami-syed/labs/week-09-protein-purification/index.htmlThis lab introduced the fundamentals of protein extraction and purification workflows commonly used in synthetic biology and bioengineering. It was particularly valuable for my final project, since our system required growing and extracting GFP protein before conjugating it to magnetic microparticles. It was also useful to understand how magnetic separation and purification methods can be integrated into biological systems, as my project similarly uses magnets both to purify ligand-conjugated particles and to actively control their interactions with cells. To isolate our protein of interest, we first grew the cells and then lysed them using a combination of B-PER (Bacterial Protein Extraction Reagent) and sonication, producing a lysate solution containing the total protein content of the cells.Hugoen