https://pages.htgaa.org/2026a/sami-syed/labs/week-10-mass-spectrometry/index.htmlThis week we did a lab at Waters Corp on Liquid Chromatography Mass Spectrometry, one of the core technologies used for modern protein characterization. Their lab was so cool!! Using enhanced Green Fluorescent Protein (eGFP) as the model system, the lab showed how proteins can be analyzed at multiple levels ranging from overall molecular weight and folding state to their exact amino acid sequence. I found it especially interesting because the workflow progressively “breaks down” the protein from an intact structure into smaller peptide fragments, revealing different layers of biological information at each stage. We also briefly explored Charge Detection Mass Spectrometry (CDMS), which can analyze extremely large biological complexes that are too massive for conventional mass spectrometry techniques.Hugoen