https://pages.htgaa.org/2026a/samudera-mukhalid/homework/week-06-hw-genetic-circuits-part-i-assembly-technologies/index.html1. Components in Phusion High-Fidelity PCR Master Mix and Their Purpose One of the main enzymes used is Phusion High-Fidelity DNA Polymerase, a DNA polymerase with an extremely low error rate (high fidelity), making it ideal for mutagenesis and cloning experiments. The main components in Phusion High-Fidelity PCR Master Mix and their functions are: Components Function Phusion DNA Polymerase Enzyme that synthesizes new DNA from primers during the extension phase. It has proofreading activity (3’→5’ exonuclease), resulting in very low replication errors. dNTPs (dATP, dTTP, dGTP, dCTP) Substrates or “building blocks” used by the polymerase to form the new DNA strand. Reaction Buffer Provides optimal chemical conditions (pH, salts, enzyme stability) for polymerase activity. Mg²⁺ ions (MgCl₂) Essential cofactor required by DNA polymerase to catalyze phosphodiester bond formation between nucleotides. Stabilizing agents Maintain enzyme stability during PCR. The master mix is typically at 2X concentration, so only primers, template DNA, and water need to be added.Hugoen