https://pages.htgaa.org/2026a/samudera-mukhalid/homework/week-09-hw-cell-free-system/index.htmlPART 1 — General Homework Questions 1. What are the main advantages of cell-free protein synthesis over in vivo methods, particularly in terms of flexibility and control of experimental variables? Provide at least two cases where CFPS is more beneficial. Cell-free protein synthesis (CFPS) offers significantly greater flexibility compared to in vivo systems because it is not constrained by cellular viability. In this system, biological components are extracted from cells, allowing researchers to directly manipulate reaction conditions such as ion concentrations (Mg²⁺, K⁺), energy molecules (ATP), and enzymatic composition. This enables precise experimental control without interference from complex cellular regulatory networks. Furthermore, CFPS operates as an open system, meaning components can be added or removed dynamically during the reaction, enhancing experimental tunability. CFPS is particularly advantageous in several scenarios, including:Hugoen