https://pages.htgaa.org/2026a/sara-gaviria-escobar/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains all the core reagents necessary for accurate and efficient DNA amplification: Phusion DNA Polymerase: A high-fidelity enzyme with 3′→5′ exonuclease proofreading activity that minimizes errors during DNA synthesis, especially important for mutation-based cloning (NEB, 2023). dNTPs (deoxynucleotide triphosphates): Provide the nucleotide building blocks (A, T, G, C) for DNA strand elongation. Reaction Buffer (with Mg²⁺): Maintains the ionic strength and conditions needed for optimal enzyme activity and DNA strand stability. Stabilizers & enhancers: Help maintain enzyme performance across temperature ranges and buffer pH changes during thermocycling. (New England Biolabs (NEB), 2023).Hugoen